概述
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产品编号 ： SY064 优 |
抗体来源： Mouse |
应用: WB，IF, IHC |
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反应性: H |
MW：170kD(Observed） |
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Western Blot
Western blot analysis of Hela with EGFR mAb diluted at 1:1,000.
IF
IF analysis of Hela with SY064(Left) and DAPI (Right) diluted at 1:100.
IHC
Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using EGFR （SY064）Mouse mAb diluted at 1:500.
产品描述
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蛋白名称 |
EGFR |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The EGFR mouse monoclonal antibody can detect endogenous EGFR proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB1:1,000 IF 1;100-200 IHC 1:200-500 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
170kD |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody |
背景
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The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues. |
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