概述
|
产品编号 ： SY075 优 |
抗体来源： Mouse |
应用: WB, IHC, IF |
|
反应性: H |
MW：53kD(Observed） |
|
Western Blot
Western blot analysis of 293T, with p53 mAb diluted at
1) 1:1,000
2) 1:2,000
Immunohistochemistry
IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb（6C4）diluted at 1:200.
IF
IF analysis of Hela with SY075(Left) and DAPI (Right) diluted at 1:100.
产品描述
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蛋白名称 |
p53 |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The p53 mouse monoclonal antibody can detect endogenous p53 proteins. |
|
存储 |
Store at -20°C. Stable for one year from the date of shipment. |
|
组成 |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
|
稀释比例 |
WB 1:2,000 IHC 1:200 IF 1:100-200 (Optimal dilutions should be determined by the end user) |
|
浓度 |
1 mg/ml |
|
实测条带 |
53kD |
|
克隆性 |
Monoclonal |
|
同种型 |
IgG1 |
|
别名 |
Cellular tumor antigen p53 antibody, Tumor protein p53 antibody, TRP53 antibody, Antigen NY-CO-13 antibody |
背景
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p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. |
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