概述
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产品编号 ： SY089 优 |
抗体来源： Mouse |
应用: WB, IHC |
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反应性: H, M, R |
MW：47kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates with NSE mAb diluted at 1:3,000.
Immunohistochemistry
IHC staining of Human small cell carcinoma of lung tissue with NSEmouse mAb(13E2) diluted at 1:200.
产品描述
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蛋白名称 |
NSE |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The NSE mouse monoclonal antibody can detect endogenous NSE proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:2,000 IHC 1:200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
47kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Enolase 2 antibody, Neuron specific gamma enolase antibody, ENOG antibody, Eno 2 antibody |
背景
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Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. |
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