概述
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产品编号 : SY098 优 |
抗体来源: Mouse |
应用: WB, IF |
|
反应性: H, M, R |
MW:48kD(Observed) |
|
Western Blot
Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, with FH Mouse mAb diluted at 1:3,000.
Western Blot
Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, with FH Mouse mAb diluted at 1:3,000.
IF
IF analysis of Hela with SY098 diluted at 1:100.
产品描述
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蛋白名称 |
FH/Fumarase |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The FH mouse monoclonal antibody can detect endogenous FH proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
|
组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
|
稀释比例 |
WB 1:3,000 IF 1:100-200 (Optimal dilutions should be determined by the end user) |
|
浓度 |
1 mg/ml |
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实测条带 |
48kD(Observed) |
|
克隆性 |
Monoclonal |
|
同种型 |
IgG1 |
|
别名 |
Fumarate hydratase mitochondrial antibody, MCL antibody, MCUL 1 antibody |
背景
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Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. |
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