概述
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产品编号 ： SY101 优 |
抗体来源： Mouse |
应用: WB, IP,IHC |
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反应性: H |
MW： 35-38kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela, 2) 293T, with CA IX Mouse mAb diluted at 1:5,000.
IP
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : SY101 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,0000.
IHC
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using CA IX/Carbonic Anhydrase IX（SY101）Mouse mAb diluted at 1:200.
产品描述
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蛋白名称 |
CA IX/Carbonic Anhydrase IX |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The CA IX/Carbonic Anhydrase IX mouse monoclonal antibody can detect endogenous CA IX/Carbonic Anhydrase IX proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:3,000 IP 1:200 IHC 1:100-200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
35-38kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
CA9 antibody, Carbonic anhydrase 9 antibody, Renal cell carcinoma associated antigen G250 antibody |
背景
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The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors. |
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