概述
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产品编号 ： SY167 |
抗体来源： Mouse |
应用: WB, IF,IP |
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反应性: H, R, M, Mk, Dg, C, Hm, Rb, Sh |
MW：55kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela ,2) Mouse Kidney tissue, 3) Rat Heart tissue with SY167 diluted at 1:5,000.
IP
1、Input: Mouse Brain Tissue Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : SY167 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000
产品描述
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蛋白名称 |
β-tubulin Mouse mAb(Mix-mA™) |
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来源 |
A Mouse Mixture monoclonal antibody is prepared by immunizing recombinant protein. |
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特异性 |
The β-tubulin Mouse mAb(Mix-mA™) can recognize endogenous β-tubulin proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000-10,000 IHC 1:200 IP 1:200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
55kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain |
背景
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Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. |
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