Flag-Tag Mouse mAb(2C5)-HRP conjugated

产品编号 ： SY034

抗体来源： Mouse

应用: WB

反应性: N/A

MW： N/A

蛋白名称

Flag-Tag Mouse mAb(2C5)-HRP conjugated

来源

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. Antibody conjugated to HRP.

特异性

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins

存储

Store at -20°C. Stable for one year from the date of shipment.

组成

in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

稀释比例

WB  1:5,000-10,000

(Optimal dilutions should be determined by the end user)

浓度

1 mg/ml

实测条带

N/A

克隆性

Monoclonal

同种型

IgG1

别名

DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.