概述
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产品编号 ： SY169 |
抗体来源： Mouse |
应用: WB, IP, IF |
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反应性: N/A |
MW： N/A |
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Western Blot
Western blot analysis of His-Tag recombinant protein with SY169 diluted at 1:10,000
1) His-MBP recombinant protein
2) His-Tag transfected 293T.
产品描述
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蛋白名称 |
His-Tag |
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来源 |
A Mouse Mixture monoclonal antibody is prepared by immunizing recombinant protein. |
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特异性 |
The His-Tag Mouse mAb(Mix-mA™) can recognize His-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000 IP 1:200 IF 1:200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
6*His, 6 His tag antibody |
背景
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A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
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