Flag-Tag Mouse Monoclonal Antibody(1C1)
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Flag-Tag Mouse Monoclonal Antibody(1C1)

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概述

产品编号 SY661

抗体来源 Mouse

应用: WB

反应性:  ALL

MW N/A

 

Western Blot

 

Western blot analysis of Flag Recombinant Protein using Flag (SY661)Mouse mAb diluted at 1:10000.

产品描述

蛋白名称

Flag-Tag Mouse Monoclonal Antibody(1C1)

来源

A Mouse Monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

特异性

The Flag-Tag  Mouse Monoclonal antibody can recognize Recombinant Flag-Tag proteins.

存储

Store at -20°C. Stable for one year from the date of shipment.

组成

in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol

稀释比例

 WB 1:5,000

(Optimal dilutions should be determined by the end user)

浓度

1 mg/ml

实测条带

N/A

克隆性

Monoclonal

同种型

IgG1

别名

Green Fluorescent Protein antibody


背景

 The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

 

 

 

 

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