概述
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产品编号 : SY661 |
抗体来源: Mouse |
应用: WB |
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反应性: ALL |
MW: N/A |
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Western Blot
Western blot analysis of Flag Recombinant Protein using Flag (SY661)Mouse mAb diluted at 1:10000.
产品描述
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蛋白名称 |
Flag-Tag Mouse Monoclonal Antibody(1C1) |
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来源 |
A Mouse Monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The Flag-Tag Mouse Monoclonal antibody can recognize Recombinant Flag-Tag proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol |
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稀释比例 |
WB 1:5,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Green Fluorescent Protein antibody |
背景
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The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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