Flag-Tag Rabbit pAb

产品编号 ： SY1112

抗体来源： Rabbit

应用: WB

反应性:  Species independent

MW： N/A

蛋白名称

Flag-Tag

来源

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

特异性

The Antibody can detects recombinant Flag-Tag proteins.

存储

Store at -20°C. Stable for one year from the date of shipment.

组成

in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

稀释比例

 WB 1:5,000-20,000

(Optimal dilutions should be determined by the end user)

浓度

1 mg/ml

实测条带

N/A

克隆性

Monoclonal

同种型

IgG

别名

DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

 The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.