概述
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产品编号 ： SY576 |
抗体来源： Rabbit |
应用: WB, IHC |
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反应性: H, R, M |
MW：43kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate, 4) Rat Brain Tissue Lysate using β-actin （SY576）Rabbit pAb diluted at 1:5000.
IHC
Immunohistochemical analysis of paraffin-embedded Human Heart Tissue using β-actin （SY576）Rabbit pAb diluted at 1:200.
IHC
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using β-actin SY576）Rabbit pAb diluted at 1:200.
产品描述
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蛋白名称 |
β-actin |
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来源 |
Rabbit Polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The β-actin Rabbit Polyclonal antibody can detect endogenous β-actin proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:2,000-5,000 IHC 1:100-200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
43kD(Observed） |
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克隆性 |
Polyclonal |
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同种型 |
IgG |
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别名 |
actin antibody, b-actin antibody, beta-actin antibody |
背景
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β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues. |
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