概述
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产品编号 ： SY138 |
抗体来源： Mouse |
应用: WB, IP, IF,IHC |
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反应性: H, R, M |
MW：65kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with SY138 diluted at 1:2000.
IP
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : SY138 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000.
IF
IF analysis of Hela with SY138(Left) and DAPI (Right) diluted at 1:100.
IHC
Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 （SY138）Mouse mAbdiluted at 1:500.
产品描述
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蛋白名称 |
NFκB p65 |
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来源 |
Mouse monoclonal antibody is prepared by immunizing recombinant protein. |
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特异性 |
The NFκB p65 mouse monoclonal antibody can detect endogenous NFκB p65 proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
65kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Nuclear Factor NF Kappa B p65 Subunit antibody, p65 NF kappaB antibody, Transcription Factor p65 antibody |
背景
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NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection. |
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