概述
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产品编号 ： SY162 |
抗体来源： Mouse |
应用: WB, IP, IF, IHC |
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反应性: H |
MW：170kD(Observed） |
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Western Blot
Western blot analysis of 1) A431 ,2) Hela, with SY162 diluted at 1:2,000.
IP
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : SY162 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific(S003), 1:5,000.
IF
IF analysis of Hela with SY162(Left) and DAPI (Right) diluted at 1:100.
产品描述
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蛋白名称 |
EGFR |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The EGFR antibody can recognize endogenous EGFR proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:1,000-3,000 IP 1:200 IF 1:100-200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
170kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody |
背景
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The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. |
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