概述
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产品编号 ： SY164 |
抗体来源： Mouse |
应用: WB, IF, IHC |
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反应性: H, R, M |
MW：48kD(Observed） |
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Western Blot
Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with SY164 diluted at 1:2,000.
IF
Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with SY164 diluted at 1:2,000.
IHC
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（SY164）Mouse mAb diluted at 1:200.
产品描述
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蛋白名称 |
eIF4A1 |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The eIF4A1 antibody can recognize endogenous eIF4A1 proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
48kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A |
背景
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The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression. |
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