概述
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产品编号 ： SY001 |
抗体来源：Mouse |
应用: WB, IP, IF |
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反应性: N/A |
MW：N/A |
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Western Blot
1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
IP
IP antibody use：5ug Flag Mouse IgG1 per ml Lysate，WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with Flag-tag fusion protein
3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4、IP (transfected 293+anti- Flag mAb+ Protein G agarose)
5、IP (transfected 293+Protein G)
6、Recombinant protein (E.coli)
IF
IF analysis of 293 cells transfected with a Flag-tag protein, using SYbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)
产品描述
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蛋白名称 |
Flag-Tag |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB1:5,000 IP 1:200 IF 1:2,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
DDDDK Tag antibody, DYKDDDDK Tag antibody |
背景
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The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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