概述
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产品编号 ： SY043 |
抗体来源：Mouse |
应用: WB, IHC, IF |
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反应性: H, R, M, Mk, Dg, Ch, Hm, Rb, Sh, Pg, Insect, Yeast |
MW：55kD(Observed） |
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Western Blot
Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.
Immunohistochemistry
IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
IF
IF analysis of Hela with TDY043(Left) and DAPI (Right) diluted at 1:100.
产品描述
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蛋白名称 |
β-tubulin |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The β-tubulin mouse monoclonal antibody can detect endogenous β-tubulin proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000 IHC 1:200 IF 1:100-200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
55kD |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
beta tubulin antibody, TUBB antibody |
背景
背景
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Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. |
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