概述
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产品编号 ： SY005 |
抗体来源：Mouse |
应用: WB, IP, IF |
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反应性: N/A |
MW：N/A |
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Western Blot
1ug V5 fusion protein+ Primary antibody dilution at
1、1:5,000
2、1:10,000
IP
IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with V5-tag fusion protein
3、IP(untransfted 293+anti-V5 mAb+Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-V5 mAb+ Protein G agarose)
6、IP (transfected 293+Protein G)
7、Recombinant protein (E.coli)
IF
IF analysis of 293 cells transfected with a V5-tag protein, using TDYbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)
产品描述
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蛋白名称 |
V5-Tag |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The V5 tag antibody can recognize C-terminal, internal, and N-terminal V5-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000 IP 1:200 IF 1:1,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
GKPIPNPLLGLDST tag antibody, rev epitope tag antibody |
背景
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The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. |
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