概述
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产品编号 ： SY042 |
抗体来源： Mouse |
应用: WB, IHC |
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反应性: H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh, Insect, Yeast |
MW：37kD(Observed） |
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Western Blot
Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb(2B8) diluted at 1:10,000.
Western Blot
Western blot analysis of Chicken Muscle (1), Hamster Lung (2), Pig Heart(3), with GAPDH mouse mAb(2B8) diluted at 1:10,000.
产品描述
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蛋白名称 |
GAPDH |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The GAPDH mouse monoclonal antibody can detect endogenous GAPDH proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000 IHC 1:200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
37kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
G3PDH antibody, Glyceraldehyde 3 phosphate dehydrogenase antibody, MGC102544 antibody |
背景
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Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
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