概述
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产品编号 ： SY044 |
抗体来源： Mouse |
应用: WB, IHC |
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反应性: H, R, M |
MW：29kD(Observed） |
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Western Blot
Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000.
Immunohistochemistry
IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb（12D10）diluted at 1:200
产品描述
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蛋白名称 |
PCNA |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The PCNA mouse monoclonal antibody can detect endogenous PCNA proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000 IHC 1:200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
29kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
Proliferating Cell Nuclear Antigen antibody, DNA polymerase delta auxiliary protein antibody, PCNAR antibody |
背景
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PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
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