概述
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产品编号 ： SY048 |
抗体来源： Mouse |
应用: WB, IP, IF ,IHC |
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反应性: H, R, M |
MW：52kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with SY048 diluted at 1:5,000.
IHC
Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using α tubulin （SY048）Mouse mAb diluted at 1:500.
IP
1、Input: Mouse Brain Tissue Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : SY048 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000
IF
IF analysis of Hela with SY048(Left) and DAPI (Right) diluted at 1:100.
产品描述
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蛋白名称 |
α-tubulin |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The α-tubulin mouse monoclonal antibody can detect endogenous α-tubulin proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
52kD(Observed） |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain |
背景
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Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin. |
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