概述
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产品编号 ： SY055 |
抗体来源：Mouse |
应用: WB, IF, IP |
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反应性: H, R, M, Yeast |
MW：15kD(Observed） |
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Western Blot
Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with SY055 diluted at 1:5,000.
IF
IF analysis of Hela with DAPI (Left) and SY055(Right) diluted at 1:200.
IP
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : SY055 1:2,000
Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000
产品描述
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蛋白名称 |
Histone H3 |
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来源 |
Mouse monoclonal antibody is prepared by immunizing recombinant protein. |
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特异性 |
The Histone H3 mouse monoclonal antibody can detect endogenous Histone H3 proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:2,000-5,000 IP 1:200 IF 1:200 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
15kD(Observed） |
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克隆性 |
Polyclonal |
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同种型 |
IgG1 |
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别名 |
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
背景
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Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
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