概述
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产品编号 ： SY003 |
抗体来源：Mouse |
应用: WB, IF, IP |
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反应性: N/A |
MW： N/A |
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Western Blot
0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000.
IF
IF analysis of 293 cells transfected with a HA-tag protein, using SYbio anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA)
IP
IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with HA-tag fusion protein
3、IP(untransfted 293+anti-HA mAb+Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-HA mAb+ Protein G agarose)
6、IP (transfected 293+Protein G)
7、Recombinant protein (E.coli)
产品描述
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蛋白名称 |
HA-Tag |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The HA tag antibody can recognize C-terminal, internal, and N-terminal HA-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB 1:5,000 IP 1:200 IF 1:1,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
hemagglutinin antibody |
背景
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Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection. |
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