概述
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产品编号 ： SY004 |
抗体来源：Mouse |
应用: WB, IP, IF |
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反应性: N/A |
MW：N/A |
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Western Blot
2ug His fusion protein+ Primary antibody dilution at
1、1:5,000
2、1:10,000
Western Blot
1、Multi-Tag Recombinant protein
2、293T transfected with His recombinant protein
3、Insect cell trandfected with His recombinant protein
IP
IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with His-tag fusion protein
3、IP (untransfected 293+anti-His mAb+ Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-His mAb+ Protein G agarose)
6、Recombinant protein (E.coli)
IF
IF analysis of 293 cells transfected with a His-tag protein, using SYbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His)
产品描述
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蛋白名称 |
His-Tag |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB1:5,000 IP 1:200 IF 1:1,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
6 His tag antibody |
背景
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A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
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