概述
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产品编号 : SY006 |
抗体来源:Mouse |
应用: WB, IP, IF |
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反应性: N/A |
MW:N/A |
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Western Blot
1ug VSV-G fusion protein+ Primary antibody dilution at
1、1:5,000
2、1:10,000
IP
IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with VSV-G-tag fusion protein
3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)
6、IP (transfected 293+Protein G)
7、Recombinant protein (E.coli)
IF
IF analysis of 293T cells transfected with a VSV-G-tagged protein, using SYbio VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G)
产品描述
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蛋白名称 |
VSV-G-Tag |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The VSV-G-tag antibody can recognize C-terminal, internal, and N-terminal VSV-G-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB1:5,000 IP 1:200 IF 1:1,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
VSV tag antibody, VSVG tag antibody |
背景
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Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation. |
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