VSV-G-Tag Mouse mAb(8D6)
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VSV-G-Tag Mouse mAb(8D6)

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概述

产品编号 SY006

抗体来源Mouse

应用:  WB, IP, IF

反应性:      N/A

MWN/A

 

 

 

 

Western Blot

 

 

1ug VSV-G fusion protein+ Primary antibody dilution at

1、1:5,000

2、1:10,000

IP

 

 

IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with VSV-G-tag fusion protein

3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)

6、IP (transfected 293+Protein G)

7、Recombinant protein (E.coli)

IF

 

 

IF analysis of 293T cells transfected with a VSV-G-tagged protein, using SYbio VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G)

产品描述

蛋白名称

VSV-G-Tag

来源

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

特异性

The VSV-G-tag antibody can recognize C-terminal, internal, and N-terminal VSV-G-tag fusion proteins.

存储

Store at -20°C. Stable for one year from the date of shipment.

组成

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

稀释比例

 WB1:5,000 IP 1:200 IF 1:1,000

(Optimal dilutions should be determined by the end user)

浓度

1 mg/ml

实测条带

N/A

克隆性

Monoclonal

同种型

IgG1

别名

VSV tag antibody, VSVG tag antibody


背景

Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.

 

 

 

 

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