概述
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产品编号 : SY017 |
抗体来源:Mouse |
应用: WB |
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反应性: N/A |
MW:N/A |
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Western Blot
2ug S-Tag fusion protein+ Primary antibody dilution at
1、1:5,000
2、1:10,000
产品描述
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蛋白名称 |
S-Tag |
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来源 |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. |
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特异性 |
The S tag antibody can recognize S-tag fusion proteins. |
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存储 |
Store at -20°C. Stable for one year from the date of shipment. |
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组成 |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
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稀释比例 |
WB1:5,000 (Optimal dilutions should be determined by the end user) |
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浓度 |
1 mg/ml |
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实测条带 |
N/A |
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克隆性 |
Monoclonal |
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同种型 |
IgG1 |
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别名 |
KETAAAKFERQHMDS tag antibody |
背景
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S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.The amino acid sequence of the S-tag is: KETAAAKFERQHMDS conjugated to KLH. S- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins. |
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