Flag-Tag Rabbit pAb

产品编号 ： SY021

抗体来源： Rabbit

应用:  WB

反应性:    N/A

MW：N/A

蛋白名称

Flag-Tag

来源

Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

特异性

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

存储

Store at -20°C. Stable for one year from the date of shipment.

组成

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

稀释比例

 WB1:5,000

(Optimal dilutions should be determined by the end user)

浓度

1 mg/ml

实测条带

N/A

克隆性

Polyclonal

同种型

IgG

别名

DDDDK Tag antibody, DYKDDDDK Tag antibody

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.